Antimicrobial peptide (AMPs) a kind of small molecule protein from various organisms, which is stable and has an obvious antimicrobial effect,s and does not produce drug resistance. Non-ruminants lack phytase and cannot directly utilize phosphorus in phytate. The addition of phytase preparation to animal feed can promote the use of phosphorus in feed, and also reduce the pollution of phosphorus to the environment.
AMPs and phytase can be added to animal feed as two highly effective feed additive proteins to prevent feed spoilage and promote animal digestion and absorption. However, the yield of natural target product-producing bacteria is very low and not easy to isolate and purify. Therefore, Lifeasible has improved the yield of AMPs and phytases by multi-system expression and genetic engineering of bacteria to further help customers achieve industrial production.
We use genetically engineered bacteria to produce AMPs. The AMPs we obtained are highly purposeful, high-yield, scalable, and low-cost lower, wide applications, which can help you develop new green, ecological, safe, and efficient feed additives as an alternative to antibiotics.
We can construct a recombinant bioreactor for efficient expression of phytase and industrialize cheap production of phytase through the fermentation method. To solve the current problem of the high cost of phytase production and difficulty in promoting an application in feed.
- Joint development services
AMPs and phytases have a very important role and urgent need in the animal breeding industry. In addition to developing them separately, we also use genetic engineering technology to introduce synthetic AMPs genes and phytase genes into yeast expression vectors. By inducing heterologous expression of the target genes in yeast, the production of AMPs and phytase is increased.
- The AMPs Odorranain gene was synthesized artificially, the Pichia pastoris expression vector was constructed, and a recombinant Pichia pastoris strain with broad-spectrum antibacterial activity was obtained. In addition, we will also select BMMY medium to significantly increase the expression of yeast Odorranain.
- We used recombinant Kluyveromyces lactis genetically engineered bacteria producing AMPs PSI as the starting strain and established the process of high-density fermentation in 10-100 L fermenters and product isolation and purification method based on shake flask culture.
- We used the phytase strain Penicillium glabrum as the starting strain, amplified the phytase gene fragment by PCR, cloned it into the yeast expression vector, and constructed a yeast recombinant plasmid. We successfully achieved the expression of phytase in Kluyveromyces lactis by transferring the plasmid into Kluyveromyces lactis cells.
Livestock farming is moving towards high density and intensification, and the demand for feed has increased greatly. However, during the transportation and storage of feeds, they are easily contaminated by bacteria and thus deteriorate, which not only poses a health risk to animals after consumption but also causes certain economic losses. Therefore, there is a trend to find a new bacterial inhibitor to replace the existing preservatives to prevent feed contamination, and Lifeasible can help you to develop AMPs and phytases to achieve this purpose. Please feel free to contact us to discover more about our services.
For research use only, not intended for any clinical use.
