Rhizobia not only provide nitrogen fertilizer for plants but also add nitrogen to the soil. As the rhizobia grow with the green manure crop, it converts the nitrogen in the air into nitrogen that can be used by the green manure crop and fixed in the plant. In this way, each harvest is a miniature fertilizer processing plant. When the plants grow up, all the nitrogen enters the soil by returning the whole plant to the soil, making the soil's organic matter content and nitrogen content increase.
Many Fabaceae family plants can be inoculated with appropriate rhizobia, which eliminates the need for chemical fertilizers and increases plant yields. Lifeasible can select rhizobia strains that meet the needs of organic fertilizer production and additive development and production through a variety of methods, including natural selection, cross selection, mutagenesis breeding, and genetic level breeding.
| Methods |
Details |
| Natural selection |
We can select strains with good symbiotic match and strong symbiotic nitrogen fixation through symbiotic matching test and rhizobial reassortment test using the double bowl culture method. |
| Hybridization selection |
We use two or more strains with large differences in genetic traits to recombine genes between their strains through sexual crosses, paralogous crosses, and genetic transformation to concentrate the good traits of the parents in the offspring. |
| Mutagenesis breeding |
We use technologies such as laser mutagenesis, microwave mutagenesis, chemical mutagenesis, and protoplast fusion to select the ideal strain for our customers. |
| Gene-level breeding |
We apply marker genes such as luxAB, GFP, and RFP that can be stably inherited exogenous genes and do not affect the symbiotic nodule fixation behavior. It has the advantage of being fast, easy, and accurate. It can be used to evaluate the competitive nodulation ability of the strains. |
Selection and breeding of acid-tolerant rhizobia strains
Preparation and analysis of rhizobacterial agents
- Preparation of rhizobacterial agents. Mycorrhizal agents for seed dressing and direct application into the soil.
- Mycorrhizal agent quality testing. Counting of viable bacteria of rhizobia.
Development of high-efficiency rhizobial biofertilizer preservation material
The rhizobacterial agent is a bacterial fertilizer that refers to microbial products containing large amounts of rhizobia that can fix nitrogen in the air. It does not contain a large number of nutrients required by the crop itself, but rhizobia can invade root cells through root hairs to form rhizomes. In addition to providing special rhizobia selections, we are also focusing on enhancing the development of highly effective rhizobia biofertilizer preservation materials to improve the shelf life of rhizobia and their ability to survive in soil.
Rhizobia are symbiotic with green manure crops, fixing nitrogen in the air to supply nitrogen nutrients to crops, promoting crop yield increase, and improving soil fertility. Lifeasible has developed and established an efficient rhizobia selection technology platform, and we have a well-developed selection procedure, which makes the breeding cycle shorter and can help you reduce production costs and get high-quality target strains faster. Please contact our staff for target strain selection programs.
For research use only, not intended for any clinical use.
