Yeast is a single-celled fungal microorganism with about 56 genera and 500 species known. The quality control of yeast determines the quality of fermentation products, including fermented feed and fertilizer. Therefore, the selection and breeding of yeast with excellent performance is a long-term and difficult task for feed and fertilizer producers and researchers.
Genetic engineering breeding technology is directional and can breed, improve and enhance the flavor of yeast, etc. Lifeasible uses bioengineering technology to select and construct a large number of yeast strains that are resistant to high temperatures, highly adaptable, and rich in target products. We can give yeast new characteristics without changing its original properties. We can effectively utilize the nutrients from the fermentation process, reduce the number of unfavorable by-products produced, and improve the fermentation performance of yeast strains.
Solutions we offer
| Services |
Details |
| Mutagenesis breeding service for yeast |
We used various mutagens such as nitrosoguanidine, ultraviolet light, nitrite, and lithium chloride to compound the yeast strain for mutagenesis treatment. The treated suspensions are subjected to primary screening with special plates, and then the fermentation supernatant is taken for re-screening. After several mutagenesis treatments, we can screen out strains with specific traits from many mutant strains. |
| Cross-breeding service for yeast |
We achieve the purpose of combining and optimizing the characteristics of industrial strains through the genetic isolation and recombination of the two parents, and we screen the target strains for our customers.
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| Fusion breeding service for yeast |
Preparation and regeneration of protoplasts
Our application of protoplast fusion technology for yeast breeding generally includes a screening of fusion parent strains, determination of fusion sub-screening methods, preparation of protoplasts, a fusion of protoplasts, screening and identification of fusion subsets, and testing of production performance of fusion strains. |
Haploid preparation
- Preparation of protoplasts
- Regeneration of protoplasts
- Calculation of protoplast formation rate and regeneration rate
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Protoplast fusion
- Chemical fusion method
- Electrofusion method
- Laser-induced fusion method
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Screening of fusion substrates
- Use of nutrient-deficient phenotypes as genetic markers
- Inactivated donor method
- Selection of fusions using fluorescent staining
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Our success cases
- Construction of high-temperature and high-concentration fermentation strains
- Selection and breeding of autolysis-resistant yeast strains
- Construction of saccharolytic yeast
- Construction of saprophytic yeast
- Construction of flocculating yeast
- Selection and breeding of aroma-enhancing yeast
- Selection and breeding of yeast with a strong ability to degrade malic acid
Wide range of applications
- Effective utilization of dextrins
- Beta-glucan catabolism
- Reduction of diacetyl production
- Reduction of H2S production
- Reduction of higher alcohol production
- Solve the problem of high alcohols and low esters
- Improvement of fermentation performance
Breeding yeast using genetic engineering is a widely used method today. Lifeasible uses yeast mutagenesis, cross-breeding, and fusion breeding techniques to target yeast breeding, which will result in better quality yeast strains and help you produce better quality, better-flavored products. Please contact our staff to learn how we can help you select and develop yeast strains for feed and fertilizer production.
For research use only, not intended for any clinical use.
