Glutathione (GSH) is an important physiologically active peptide with a variety of important physiological functions in living organisms. In particular, it plays a key role in maintaining a suitable redox environment in living organisms. There have also been many studies in terrestrial and aquatic animal farming, all of which have shown that GSH can well improve the immune performance of the organism, antioxidant properties, and increase its resistance to pathogenic bacterial infections and ammonia and nitrite stress.
To meet the needs of our customers for GSH, Lifeasible offers a complete development service for more cost-effective microbial fermentation for GSH production. This includes high yield strain selection (mutagenesis breeding and genetically engineered bacteria construction) and fermentation process optimization. We aim to assist you in introducing GSH, an active ingredient with a wide range of applications, into the field of biofertilizer and organic feed.
High-yielding strain selection service
- Conventional mutagenesis breeding for the selection of high GSH yielding strains
Lifeasible uses physical or chemical methods to mutate the original strain, which is then screened and cultured in a specific medium. GSH is accumulated in large quantities in the cells of this mutated strain to achieve high GSH production.
| Selective breeding strains |
Treatment methods |
Screening substances |
- Candida utilis
- Saccharomyces cerevisiae
|
- UV, X-rays, γ-rays, and nitrosoguanidine
- Cellular protoplast fusion technique
|
Methionine, L-ethionine, DL-ethionine, 1,2,4-triazole, sodium cyanide, sulfite, amide, indophenol, etc. |
- Genetic engineering breeding to select strains with high GSH production
Lifeasible used genetic engineering techniques to construct genetically engineered bacteria with GSH synthesis activity. We can transform the gshⅠ and/or gshⅡ genes into yeast cells to obtain recombinant strains with high GSH production.
Fermentation process optimization services
- Optimization of culture medium
Lifeasible uses a central composite experimental design approach to optimize the major components of the fermentation medium. To significantly increase GSH production and cell dry weight.
- Optimization of fermentation conditions
Environmental conditions are also an important factor in the production of GSH by fermentation, and Lifeasible conducts simulations to address the environmental parameters that affect the GSH content in cells, including temperature, dissolved oxygen, pH, etc. To determine the optimal conditions for GSH production by fermentation under different conditions.
In addition to its high clinical value, GSH is increasingly used in the biofertilizer and organic feed industries related to the production of farming. Lifeasible provides services for the selection of high-yielding GSH strains and the optimization of culture conditions. We can also model the effect of fermentation production conditions on GSH production and its fermentation kinetics according to the different needs of our customers. We welcome your inquiries for any related questions of interest. If you are interested in collaborating with us, feel free to contact us to expedite your project research.
For research use only, not intended for any clinical use.
