Nitrogen-fixing Bacteria Selection and Development

Nitrogen is not only one of the most essential nutrients for plant growth but also one of the key factors in determining crop yield and quality. Biological nitrogen fixation is a process by which nitrogen-fixing microorganisms reduce nitrogen in the atmosphere that cannot be directly used by plants for ammonia. It is an inexhaustible and inexpensive source of nitrogen. Therefore, the mining and utilization of biological nitrogen fixation are of great significance to the reduction of environmental pollution and sustainable development of agriculture.

Combined nitrogen fixation is a nitrogen fixation system formed by nitrogen-fixing bacteria in the roots, stems, or leaves of higher plants without special symbiotic structures. Lifeasible provides strain culture, screening, nitrogen-fixing enzyme activity measurement, and special mutant strain screening and selection services to promote the popularity of combined nitrogen-fixing bacteria in fertilizers. Inoculation with nitrogen-fixing bacteria can not only reduce the amount of nitrogen fertilizer applied by half but also increase or maintain crop yield.

Nitrogen-fixing enzyme activity measurement service

We measure nitrogen-fixing enzyme activity in nmol (C₂H₄)·g^-1(FW)·h^-1 by measuring ethylene amount with gas chromatography. We also obtain accurate and reproducible complete data for our customers through professional data processing and image analysis.

In addition, we offer our customers a wide range of measurement methods to choose from. These include total nitrogen analysis, nitrogen balance method, isotope tracing method, and acetylene reduction method.

Strain culture and screening services

We will select suitable media to culture different strains according to the characteristics of the strains and your project needs, and screen them according to your specific needs to help you get good-quality strains as soon as possible.

Special mutant strain screening and breeding services

• Nitrogen-fixing enzyme genome forming expression, glutamate synthase gene knockout, ammonium secretion.
• nifL mutant strain, ammonium tolerant nitrogen fixation.
• Knockout of nifL and overexpression of nifA, ammonium secretion.
• amtB knockout mutant strain, ammonium secretion.

We can help you do this

• To target the main limiting factors affecting nitrogen fixation efficiency, we artificially designed non-coding RNA regulatory elements, and ammonium-tolerant ammonium-lactating modules and constructed efficient nitrogen fixation gene circuits.
• Artificially designed multi-level regulated abiotic stress tolerance module, carbon source efficient utilization module, and root surface biofilm formation module to construct an intelligent stress tolerance gene circuit related to efficient nitrogen fixation.
• Using the inter-root combined nitrogen-fixing microorganism as the chassis, research and system optimization of artificial regulatory elements, functional modules, and gene circuit adaptability are conducted.
• Develop new nitrogen fixation products suitable for modernized agricultural production methods such as mechanized seeding, integrated drip irrigation, and soilless cultivation.
• Using nitrogen-fixing microorganisms and host crops as the chassis, we will conduct research and system optimization on the suitability of artificial genetic circuits, and create an efficient and intelligent artificial inter-root combined nitrogen fixation system by coupling the nitrogen-efficient transporter module with the ammonium secretion module.

Using the combination of nitrogen fixating is important for agricultural weight loss and ecological protection. With a focus on agriculture and ecology, Lifeasible is committed to helping our customers improve and develop promising nitrogen-fixing bacteria and increase their applicability in a wide range of fertilizer production and applications. Please contact our staff for more information on the services we can offer you.