The most commonly used feed ingredients that are susceptible to oxidation are fish meal, oil, and fat-soluble vitamins. Because these components contain unsaturated fatty acids and components that are unstable to oxygen, they are easily damaged by oxidation during storage, transportation, and use. Oxidation will not only significantly reduce the palatability and stability of the feed, but its oxidation products will also disrupt the metabolism of the animal's body and produce toxic effects on the liver and kidneys. It can even lead to reduced performance of pregnant animals.
Therefore, the addition of antioxidants in feed is very necessary, it can not only prevent or delay the oxidation of these nutrients in the feed but also improve feed stability and extend the storage period of feed. The content of antioxidants in organic feeds is an important indicator of the quality of the product. To meet the needs of our customers, Lifeasible offers different antioxidant content testing services using a variety of technical methods. These include ultra-high-performance liquid chromatography (UHPLC), gas chromatography (GC), solid phase extraction (SPE) with high-performance liquid chromatography in tandem with diode array detector (HPLC-DAD), QuEChERS-ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, high-performance thin layer chromatography (HPTLC)-DPPH colorimetric-pixel analysis method, gel permeation chromatography (GPC) cleanup-GC triple quadrupole mass spectrometry (GC-MS/MS) analysis method.
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Methods |
Description |
| UHPLC |
This method has the significant advantages of fast peak speed, high sensitivity, and good separation. |
| GC |
We have established an acetonitrile extraction-GC method for the rapid and bulk determination of multiple antioxidants in feeds by creating and optimizing a pretreatment method for the determination of multiple antioxidants in feeds. The method is suitable when the number of sample batches is large. |
| SPE-HPLC-DAD |
We use SPE combined with HPLC-DAD to simultaneously detect multiple antioxidants in feeds with a stable method. |
| QuEChERS-UPLC-MS/MS |
We can establish a method for the determination of various antioxidants in feeds by using the QuEChERS method with enhanced matrix removal lipid (EMR-lipid) as the lipid removal adsorbent in combination with UPLC-MS/MS. |
| HPTLC-DPPH-pixel analysis |
We provide this method with good selectivity, low detection limit, good precision, and accuracy, with the advantages of matrix tolerance, accurate quantification, simplicity and reliability, and high analytical throughput. It is suitable for the rapid quantitative screening of various antioxidants in feed. |
| GPC-GC-MS/MS |
We provide this method for the rapid screening of phenolic antioxidants, which has the advantages of easy operation, good impurity removal, high sensitivity, precision, and good method reproducibility. |
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Detectable antioxidants |
- Octyl gallate (OG)
- 2,6-di-t-Butyl-4-hydroxymethyl phenol (Ionox- 100)
- Nordihydroguaiaretic acid (NDGA)
- 2,4,5-Trihydroxybutyrophenone (THBP)
- Butylated hydroxytoluene (BHT)
- Propyl glycolate (PG)
- Butylated hydroxyanisole (BHA)
- Tertiary-Butylhydroquinone (TBHQ)
- Dodecyl gallate (DG)
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Antioxidants in organic feeds can protect feeds from oxidative damage and deterioration. Lifeasible offers you the service of testing the content of many antioxidants in feeds. Please contact our staff to submit your assay request or for sample requirements.
For research use only, not intended for any clinical use.
