Production strains for feed and fertilizer applications may lose good traits that are important for industrial applications if genetic instability is created during the transmission process. Not to mention that various physiological and habitat stress conditions can accelerate the occurrence of genetic instability events. Therefore, when natural (wild) strains are used as feed and fertilizer engineering strains, they must be genetically stable in addition to having excellent characteristics.
Based on the above needs, Lifeasible not only selects natural (wild) strains for superior traits by breeding methods such as targeted breeding, mutagenesis, and cell fusion, but also provides customers with services to test the genetic stability of production strains at three levels. We offer a full range of selection and evaluation services to meet your needs for production strains in the feed and fertilizer fields.
- Genetic stability testing of production strains in the process of transmission and conservation
- Genetic stability testing of fermentation characteristics during fermentation production of feed and fertilizers
- Genetic stability testing of target genes and recombinant expression vectors in genetically engineered strain systems
Types of strains for which we can perform genetic stability testing
| Type |
Method |
| Lactic acid bacteria |
We can determine the genetic stability of parental strains and mutant strains by comparing the changes in their DNA fingerprints using random amplified polymorphic DNA (RAPD) technology. In addition, we will also examine the metabolism of the parental strain and the mutant strain by high-performance liquid chromatography (HPLC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) during the transmission process to evaluate their genetic stability. |
| Saccharomyces cerevisiae |
We use molecular sequencing to verify specific gene loci, and we observe and comparatively analyze the growth state of colonies, etc. We can detect the genetic stability of recombinant yeast during the transmission process. |
Other strains
(please contact our staff for special strains) |
We develop different protocols for different characteristics of different strains and analyze their genetic stability at the molecular level by observing colony characteristics and morphology, measuring physiological, biochemical, and fermentation properties, and using ERIC-PCR (BOX-PCR) and other detection techniques. |
Lifeasible can test the genetic stability of production strains during transmission and conservation at the phenotypic, biological, and molecular levels. We use a variety of tools including molecular sequencing, enterobacterial repetitive intergenic consensus (ERIC) PCR, BOX-A1R-based repetitive extragenic palindromic (BOX) PCR assays, and HPLC. We have established a full range of evaluation systems to meet your different needs for production strains in the feed and fertilizer fields. If you are interested in our services or have a strain that needs to be tested, please feel free to contact us to develop a plan to assist you in obtaining professional and accurate test results as soon as possible.
For research use only, not intended for any clinical use.
