Nisin Fermentation

Nisin is a natural biological antimicrobial peptide produced by the fermentation of naturally occurring Lactococcus lactis. It has a broad-spectrum antibacterial effect and can effectively inhibit the growth and reproduction of most gram-positive bacteria and their budding spores. Especially for common bacteria such as Staphylococcus aureus, Streptococcus hemolyticus, and Clostridium botulinum, it has an obvious inhibition effect. It can play the role of antiseptic and freshness preservation for many foods, feeds, and fertilizers. And it has good stability, heat and acid resistance, and good application prospects in feed and fertilizer additives and processing industries.

Nisin is a natural polypeptide substance, which can be digested and decomposed into amino acids by proteases (such as trypsin, a pancreatic enzyme, salivary enzyme, etc.) in animals after consumption, without microbial toxicity or pathogenic effects, so its safety is high. Lifeasible can produce recombinant strains expressing Nisin efficiently through genetic engineering methods to meet customers' needs.

We developed a lactic acid bacteria expression system from a Nisin-producing Lactococcus lactis strain. The system is induced by Nisin and is used to express exogenous genes, especially Nisin-like bacteriocins which usually obtain better expression. This provides the possibility to prepare bacteriocins in large quantities.

• Selection service for induction of high yielding strains of Nisin

In addition, we also use physicochemical and other mutagenesis methods to breed and screen high yield, high activity strains of Nisin and strains for special purposes. For example, strains of Lactobacillus with no drug resistance factor, strains of Lactobacillus that have good flavor and promote maturation of fermented foods, strains of Lactobacillus that produce special enzyme systems, Lactobacillus beads with high oxygen tolerance, and strains of Lactobacillus with adhesion and colonization ability. If you have special needs, you can also contact us for special strain customization service.

• Nisin's analytical testing service

We use high-performance liquid chromatography (HPLC) to test the product potency, overcoming the drawbacks of the traditional agar diffusion method. We can improve the efficiency of product testing with high accuracy and without sample destruction. We can perform stability profiling of Nisin samples to detect product potency, which can reflect the essence of potency decline to a certain extent. In addition, our HPLC method allows our customers to quickly and accurately determine the potency of Nisin in the manufacturing process and thus optimize its process parameters.

Nisin is an internationally recognized natural biological preservative with the advantages of high efficiency, safety, and non-toxic side effects. Lifeasible aims to promote the application of Nisin in the livestock breeding industry, which can reduce the use of antimicrobials and chemical additives and facilitate the production of safe food. In addition, Nisin is reported to kill at least 100 species of bacteria. Using genetic engineering technology, we can construct a bioreactor for efficient expression of Nisin, solving the problem of its low content in natural organisms and expensive production cost, providing our customers with an effective and safe new way of antibacterial. If you are interested in the services we offer or have any questions, please feel free to contact us.