The good antibacterial properties of Bacillus subtilis originate from the production of various antimicrobial substances including lipopeptides, phospholipids, bacteriocins, proteins, glucanases, chitinases, polyenes, phenols, etc. These substances have a wide spectrum of antimicrobial activity, simple structure, and stable properties to inhibit the normal growth of fungi, bacteria, and viruses. The isolation and purification of Bacillus subtilis antibacterial active substances and their development have become a hot research topic in feed and fertilizer and additive processing improvement.
The important antimicrobial peptide-producing bacteria is Bacillus subtilis, so more and more researchers put the target of antimicrobial peptide production in Bacillus subtilis. To meet the needs of researchers and customers in the production field for deeper discovery of new active compounds, Lifeasible can provide you with the isolation, extraction, and development of active components (antimicrobial peptides or other antibacterial components) of Bacillus subtilis. In addition to working with strains isolated in their natural environment, we can also develop active ingredients for mutagenized strains in combination with our existing technology platform to meet your diverse analytical and production needs.
Bacillus subtilis active ingredient extraction, isolation, and identification services
- We first obtain strains with high yields of the target active ingredient through compound mutagenesis. Further primary and secondary screening is performed to obtain mutant strains with good active ingredients and stable genetic performance.
- If your target active ingredient is antibacterial, then we will conduct an antibacterial test for the extracted and isolated active ingredient to prove its significant antibacterial effect.
- The extract is then analyzed by Tricine-SDS-PAGE gel electrophoresis to detect the molecular weight of the active substance contained in that extract.
- The crude extract was separated and purified using silica gel column chromatography and macroporous adsorbent resin to obtain a single fraction, and its antibacterial activity was detected.
- The purified fraction was further analyzed using high-performance liquid chromatography (HPLC) to detect the content of the fraction.
- Finally, the main components were identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis and data comparison.
Bacillus subtilis active ingredient development services
After separation, extraction, purification, and identification, we have helped our customers to obtain the target active ingredient from Bacillus subtilis. Depending on your needs, we can also provide subsequent development services for the target active ingredient.
The problem of drug resistance to pathogenic bacteria caused by antibiotic abuse is becoming increasingly serious, thus threatening the rapid development of farming and cultivation industries and human health. Finding brand-new drugs is an effective way to solve the problem of drug resistance. Antimicrobial peptides are becoming the focus of research in the field of animal husbandry and other fields, and have a very broad application prospect due to their wide variety, high antimicrobial activity, broad antimicrobial spectrum, and the pathogenic bacteria are not easy to produce resistance mutations, etc. Lifeasible focuses on Bacillus subtilis, the main producer of antimicrobial peptides, and provides customers with the isolation, extraction, and development of its active ingredients. Please contact our staff for your customized proposal.
For research use only, not intended for any clinical use.
