The ban on antibiotics is an inevitable trend for the healthy development of livestock farming and ecological agriculture, and the development of antibiotic alternatives has become a key issue for the healthy development of the farming industry. Enteromorpha prolifera polysaccharide (EPP) and fucoidan, with their natural, non-toxic side effects and multiple biological functions, are potential high-quality antibiotic alternatives in livestock feeds. Lifeasible provides EPP and fucoidan development services, including extraction and preparation, analytical testing, and quality improvement, to meet your research needs.
EPP development services
EPP has antibacterial, antiviral, and animal immune function-enhancing effects, and is often used in the development of animal immune enhancers and feed additives. Besides, EPP also has the characteristics of promoting plant growth and development, increasing yield, resistance to stress, and improving soil fertility, and is suitable for a variety of crops.
- Extraction process. We will use hot water extraction, ultrasonic extraction, microwave extraction, enzymatic extraction, and a combination of different methods to extract according to your research needs. This can improve the polysaccharide extraction rate and shorten the extraction time.
- Purification preparation. We perform EPP purification using columns such as diethylaminoethyl-cellulose-52 to obtain different fragments of polysaccharides to meet your needs for different structural and efficacy studies.
Fucoidan development services
Fucoidan is a complex and heterogeneous mixture of natural polysaccharides, which are used as feed additives in animal production to improve antioxidant capacity and immune function. It also has a beneficial effect on feed intake, intestinal morphology, and microflora.
| Test items |
Methods |
| Fucoidan chemical composition determination |
- Total sugar content was determined by the phenol-sulfuric acid method.
- The sulfate base content was determined by the barium chloride-gelatin turbidimetric method.
- Uronic acids content was determined by the sulfuric acid-carbazole method.
- The protein content was determined by the Komas Brilliant Blue method.
|
| UV wavelength scan of fucoidan |
The UV absorption spectra of the samples were measured at 190-400 nm using a UV-visible spectrophotometer. |
| Analysis of fucoidan monosaccharide composition |
The monosaccharide composition was analyzed by high-performance liquid chromatography with PMP-column derivatization. |
| Determination of the molecular mass of fucoidan |
The molecular mass of the samples was determined by high-performance gel chromatography. |
| Microscopic morphological analysis of fucoidan |
The surface morphology of fucoidan was observed and photographed by scanning electron microscopy under a 5 kV electric field after fixation with conductive adhesive and gold plating. |
| Particle size and potential determination of fucoidan |
Samples were placed in the sample bath of a Malvern nanoparticle size potentiostat for measurement. |
| Structural analysis of fucoidan |
The samples were scanned for analysis using Fourier infrared spectroscopy (FTIR). |
| Determination of thermal stability of fucoidan |
The thermal stability of fucoidan was determined using a simultaneous thermal analyzer. |
In addition, we can also prepare low molecular weight (<10 kDa) fucoidan by screening fucoidanase-producing strains and enzymatic digestion.
Lifeasible has also established a technology platform due to its great development potential to help customers obtain refined polysaccharide products for feed and fertilizer addition. This has far-reaching implications for promoting the development of green livestock and eco-growing industries. Please contact our staff to submit your development needs.
For research use only, not intended for any clinical use.
