Feed and fertilizer fermentation products and processes contain many different genera and species of microorganisms. The separate enumeration of different microorganisms is also an important indicator of product performance. Traditional microbial counting methods are subjective, cumbersome, time-consuming, and error-prone. It is neither possible to distinguish cells in the viable but non-culturable (VBNC) state nor to effectively quantify a single strain in a complex bacterial system.
Based on the existing foundation of microbial identification techniques, Lifeasible can provide our customers with the service of enumerating different viable microorganisms in feed and fertilizer fermentation products. We have established a variety of culturable and non-culturable methods to carry out viable enumeration of complex microorganisms, enabling targeted quantification of viable microorganisms in complex microbial agents, fermented feed and fertilizer products, etc.
We can isolate microorganisms by pre-treating the sample to remove impurities and concentrate them. The concentrated quantitative sample is coated on a slide and stained. The number of microorganisms is counted by biomicroscopic examination of the number of images on the slide coated with the quantitative sample that is stained and shows a specific state, and the corresponding survival rate is calculated.
- Metagenome binning method
This technique allows us to separate by species the contigs or scaffolds obtained by sequencing the metagenome with a mixture of sequence reads or sequence assemblies of different microorganisms.
We provide data analysis including standard and advanced analysis. You can select the corresponding analysis according to your project needs.
Standard analysis. Visualization and abundance analysis, functional annotation, species annotation, evolutionary analysis, and Circos circle mapping.
Advanced analysis. Species/functional contribution analysis, functional genomic association analysis, Swiss-Prot annotation, secreted protein prediction, Pfam annotation, gene island prediction, transporter protein annotation, CRISPR prediction, pathogen-host interactions annotation, prophage prediction, resistance gene annotation, repetitive sequence prediction, virulence factor annotation, and secondary metabolic gene cluster annotation.
We use PMA (propidium monoazide) qPCR technology that can distinguish between living and dead microorganisms, and can accurately quantify the number of live bacteria in feed and fertilizer samples. The detection sensitivity is 100 times higher than that of ordinary PCR, and the PMA treatment does not interfere with the qPCR reaction. The live bacteria counting with our provided PMA-qPCR method is fast, easy, and specific. Compared with the plate technology method, it can finish the determination of the number of live bacteria quickly, and also finish the quantitative and qualitative detection at the same time.
Lifeasible has a wealth of experience and proven technical tools, and we use a variety of methods to meet your needs for viable bacteria enumeration in fermented feed and fertilizer products in several ways. If you are interested in our services or have a specific type of strain you would like to test, please feel free to contact us for an updated proposal.
For research use only, not intended for any clinical use.
