Antimicrobial peptides (AMPs) have biological properties such as thermal stability, tolerance, pH stability, and digestive enzyme stability. They also have essential roles in stimulating wound healing, hormone, and immune regulation. And AMPs have no residue in animals, which is a green alternative feed additive to antibiotics. Therefore, this new industry of antimicrobial peptides has an extensive market application prospect.
Lifeasible uses genetically engineered bacteria to produce AMPs. The AMPs we obtained are highly purposeful, high-yield, scalable, and low-cost lower, wide applications, which can help you develop new green, ecological, safe, and efficient feed additives as an alternative to antibiotics.
Recombinant plectasin AMPs production
The main way to obtain AMPs is to isolate and purify the biologically active AMPs directly from the organism, and then study their structure and function. We can produce recombinant plectasin AMPs through the expression system of Pichia pastoris, which can overcome to some extent the disadvantages of traditional production methods, such as low content and unsuitable for large-scale production.
We can help you to realize the process scale-up pilot production, product trial, and trial run of plectasin AMPs by optimizing the high-density fermentation process of high-yielding plectasin Pichia pastoris genetically engineered bacteria and its characterization.
AMPs molecular design
- Amino acid composition and sequence alteration. We can change the sequence of amino acids without changing the amino acid species or add or subtract amino acid species to give them new properties.
- Removal of redundant structures and retention of modified active parts. Larger peptide chains and protein molecules can affect the stability, immunogenicity, etc. of antimicrobial peptides. Therefore, we can improve them by removing their redundant structures and keeping the active part.
AMPs morphology design
Different shapes of AMPs have different inhibition effects and stability, etc. We can synthesize long-chain peptides, cyclopeptides, short-chain peptides, and ultra-short peptides. Among them, the ultra-short peptide is easy to reflect hydrophobicity and positive electric property together, so it has a strong antibacterial and antiviral ability. In addition, ultra-short peptides have smaller molecular weights, so they are easier to express.
- Binding ability with bacterial cell membrane: long chain peptide > cyclopeptide > short chain peptide.
- Bactericidal activity: long-chain peptide > cyclopeptide > short-chain peptide.
- Stability: cyclopeptide > long-chain peptide > short-chain peptide.
Other AMPs expression systems we offer
- Bacillus subtilis expression system. It has the characteristics of simple culture and rapid multiplication and is easy to be produced industrially.
- Bac-To-Bac system. It has the advantages of short cycle time, easy purification, and the ability to recombine in large quantities, with wide application and high expression efficiency.
AMPs prepared by Lifeasible using various systems have the potential to become an effective antibiotic alternative and feed additive in the future. This is of great significance to improve the quality of livestock products, promote the development of green animal husbandry and ensure food safety. Please contact our staff for development proposals.
For research use only, not intended for any clinical use.
