Phytase is a general term for a class of enzymes that catalyze the hydrolysis of phytic acid and phytate to inositol and inorganic phosphate and inorganic phosphate. Adding phytase to feed can effectively decompose phytic acid present in the ration and improve the utilization of phytic phosphate in feed. Phytase plays an important role in the natural phosphorus cycle and has great potential for application as a fertilizer improver.
Phytase formulations have been widely used in livestock feed and biofertilizer production. Lifeasible provides corresponding high-quality services to meet the needs of customers in the feed and fertilizer industries for phytase activity determination and the selection and genetic modification of superior strains.
Phytase activity testing service
| Methods |
Details |
| Molybdenum yellow method (ammonium metavanadate method) |
Phytase hydrolyzes the substrate sodium phytate under certain temperature and pH conditions to produce orthophosphate and inositol derivatives. In an acidic environment, treatment with ammonium vanadylmolybdate produces a yellow complex that can be analyzed colorimetrically at a 415 nm wavelength. |
| Molybdenum blue method |
Phytase hydrolyzes the substrate sodium phytate to produce orthophosphate and inositol derivatives under certain temperature and pH conditions. In an acidic environment, treatment with ammonium molybdate produces a blue complex, which can be analyzed colorimetrically at 810 nm. The molybdenum blue method was improved by adding a reducing agent to the reaction and changing the reaction temperature and heating time to address the color instability of the molybdenum blue method. |
General test procedure
Scope of application
- Phytase products for feed additives
- Compound feed, concentrated feed, and additive premixed feed with phytase
- Biofertilizer with phytase
Phytase high-yielding strain construction service
The production of phytase is currently mainly by microbial fermentation, but most of the original phytase-producing microorganisms are not suitable for large-scale fermentation culture. Therefore, Lifeasible has been constructing high-yield strains of phytase by transferring phytase genes into appropriate strains for efficient expression through genetic engineering technology to further improve phytase production.
Currently, we have been able to successfully express the phytase gene in a variety of expression systems. These include the prokaryotic expression systems of E. coli and Bacillus megaterium, the eukaryotic expression system of Pichia pastoris, and the plant expression systems of maize, Arabidopsis thaliana, rice, and tobacco.
Genetic modification service for phytase strains
In addition, some properties of natural phytase may not be fully suitable for the requirements of practical production, such as resistance, effectiveness, etc., especially the thermal stability of phytase.
Therefore, we can also use genetic engineering technical means to genetically modify the genes at the molecular level, which is expected to lead to the improvement of these properties. We usually use point-saturation mutation techniques to modify the genes encoding phytase to induce its directed evolution, thus obtaining a large number of mutants in a short period and screening phytase that meets the production requirements.
Phytase can be used as an efficient, non-toxic, and environmentally friendly additive in the feed and fertilizer processing industries. Lifeasible provides services for testing the activity of phytase, and in addition, we aim to build highly productive, active, and stable phytase-producing genetically engineered strains. If you are interested in our services, please feel free to contact us. We look forward to cooperating with you.
For research use only, not intended for any clinical use.
