Saccharomyces cerevisiae has various effects such as improving livestock and poultry production performance, promoting intestinal development, regulating intestinal flora balance, and improving immune function. It is a green feed antibiotic that can replace antibiotics because it is non-toxic, non-polluting, and does not produce drug resistance. In addition, Saccharomyces cerevisiae is not only rich in various active substances inside the cells but also can produce active substances itself as an engineered bacterium. As a traditional industrial fermentation strain, Saccharomyces cerevisiae is widely used in fermentation, production, and addition of feeds and fertilizers and other related fields.
Microorganisms obtained by screening and isolation from natural samples are usually rarely used directly in industrial-scale production due to factors such as low yield of target compounds and weak tolerance to harsh industrial conditions. To meet the demand for high-quality strains for feed and fertilizer addition, fermentation, and other fields, Lifeasible provides technical services for specific modification of target genomic loci of Saccharomyces cerevisiae, including gene knockout, gene knock-in, targeted repair, gene replacement, and sense point mutation.
Special production strains we can construct
- Strains with low sucrose consumption
- Strains for high isoprene production
- Strains with high mevalonate production
- Strains with high ethanol production
- Strains with good tolerance to harsh fermentation conditions
- Strains with low cell wall stabilization properties facilitate the release of active substances
Technical approaches we offer
| Methods |
Details |
| Gene editing based on CRISPR/Cas9 system |
We have used the CRISPR/Cas9 system to perform operations such as knockout of the Saccharomyces cerevisiae gene, and we have achieved editing of specific genes by homologous sequences without screening tags. And we developed a CRISPR/Cas9 system for multiple knockdowns of Saccharomyces cerevisiae genome, and up to five different genomic loci can be knocked down simultaneously. Editing efficiencies of 50%-100% were achieved for a single gene up to 5 genes by co-transforming gRNA plasmids and corresponding linear homologous recombinant fragments. |
| High-throughput gene-editing technology |
We reconstruct the cell factory by modifying cell physiological functions at the genomic level in a high-throughput form to provide optimal conditions for Saccharomyces cerevisiae strain growth and product accumulation. Ultimately, we improve the production performance of the cell factory to achieve the goal of modifying and evolving Saccharomyces cerevisiae at the genomic level to meet the needs of industrialization.
Our large-scale, high-throughput modification of the Saccharomyces cerevisiae genome has resulted in a large mutation library that can be screened by us at high throughput to enable the production of specific target compounds. |
Advantages of our service offerings
- Multiple genes can be edited simultaneously, with traceless editing, precise editing, high editing efficiency, and inducible promoters, effectively reducing off-target efficiency.
- Target DNA fragments of homologous sequences can be integrated into specific sites of the genome to achieve label-free assembly of multiple genes in Saccharomyces cerevisiae in vivo.
Saccharomyces cerevisiae can be added as an additive to livestock and poultry feeds, and can also be used to make fermented feeds, thus improving feed utilization. Lifeasible achieves precise editing modifications in multiple genes by target engineering Saccharomyces cerevisiae. As a result, it does not carry resistance tags or genes coding for toxic components, ensuring the safety of livestock feed and fertilizer production. In addition, we are also developing Saccharomyces cerevisiae suitable for inclusion in feed and fertilizer, so that it can be more useful in animal and plant production. Please feel free to contact us to learn how we can improve Saccharomyces cerevisiae through genetic engineering technology to promote the healthy and stable development of farming and planting industries.
For research use only, not intended for any clinical use.
