Bacillus subtilis Breeding

Feed-grade microbial additives are a new class of feed additives that have a significant role in improving feed utilization, increasing production performance, regulating animal intestinal micro-ecological balance, and increasing immunity. Bacillus subtilis, which is currently used more in feed and animal production, has unique biological properties such as high resistance to stress, high temperature and pressure, acid and bile salts, and easy storage. From the point of view of animal production and feed industry evaluation, it can better meet the requirements of modern industrial production of microorganisms for feed.

To meet the market demand for high-quality Bacillus subtilis strains, Lifeasible can perform the selection of Bacillus subtilis strains for you. The selection of strains and the control of fermentation conditions are two complementary aspects. To maximize the production advantages of the superior strains obtained from the breeding work, we have also improved the fermentation process and the fermentation medium to compose a system that is suitable for it.

Mutagenesis breeding service for Bacillus subtilis with high chitinase production

• Chitin is widely found in the shells of crustaceans, insect carapace, and the cell walls of fungi, and has broad application prospects in the feed production industry. Feeding fish with feeds supplemented with chitinase can effectively improve feed utilization, significantly reduce fish stress, be safe and non-stimulating to fish, significantly improve the anti-inflammatory and anti-infection ability of fish, and significantly increase the immune protection rate of fish against pathogenic bacteria. Therefore, we can selectively breed Bacillus subtilis, which produces chitinase, to obtain high-yielding strains.
• We used a natural strain of Bacillus subtilis 515 with chitinase activity as a starting point and selected a mutant strain with significantly increased chitinase activity by mutagenesis treatment with UV, LiCl, and DES. We then optimized the enzyme-producing medium and fermentation process conditions using orthogonal tests to further improve the tributylase production of the mutant strain to meet your needs in practical production applications.

Selection and breeding service of Bacillus subtilis with high bacteriocin production

• Bacteriocins are an important class of natural preservatives that have attracted much attention in the field of feed production because of their high antimicrobial activity, ease of industrial production, degradability by proteases, and lack of drug resistance. The antimicrobial substance of Bacillus subtilis has a broader spectrum of bacterial inhibition, low pH requirements, good thermal stability, little effect of organic solvents on its activity, and easy handling. Therefore, we offer you a selection of Bacillus subtilis with high bacteriocin production to help you develop new natural preservatives that can be applied in feed production.

Bacillus subtilis can rapidly colonize the organism and form a good micro-ecological environment in the animal's intestine, which can enhance the animal's digestive capacity, degrade anti-nutritional factors, increase feed utilization and strengthen the animal's immunity. Lifeasible attaches great importance to the important role of Bacillus subtilis in feed production. We aim to provide you with better quality and more suitable strains for your production process by selecting and breeding Bacillus subtilis for different applications. Please feel free to contact our staff to submit your strain selection request.