Lactic acid bacteria are a group of non-spore-forming, gram-positive bacteria, which contains many species. Most of the lactic acid bacteria are not motile, a few are motile by circumferential hairs, and their bodies are often arranged in chains. This group of bacteria is extremely widespread and has a rich diversity of species, containing at least 18 genera with more than 200 species. They are extremely valuable in the field of organic feed and fertilizer development. Therefore, it is necessary to analyze the functions of their metabolic key genes and gene networks and regulate metabolic pathways with the help of gene-editing technology.
Lifeasible has been providing genome editing services for microorganisms used in fermentation for many years. For lactic acid bacteria, we can mainly provide you with the traditional λRed homologous recombination method and CRISPR/Cas9 technology to achieve highly accurate and efficient scar-free genome editing. Ultimately you can apply the improved strains in the field of feed and fertilizer fermentation to improve the traits and quality of your products.
Gene editing methods we offer
| Methods |
Traditional λRed homologous recombination method |
CRISPR/Cas9 gene-editing method |
| Description |
We use RecA and RecBCD proteins encoded by their own Rec A homologous recombination system to mediate homologous recombination of DNA, which results in allelic replacement of the target gene. By designing a homologous fusion fragment of the target gene, it is cloned into a suicide vector, which is imported into the target bacteria via splicing. An insertional mutant strain with the suicide vector integrated into the bacterial genomic target locus is screened by antibiotics. Under the second round of reverse selection pressure, only the Lactobacillus genome undergoes second homologous recombination and loses the suicide plasmid to survive. |
We identify the target genomic sequence by artificially designed sgRNA and direct Cas9 protease to efficiently cleave the DNA double-strand, creating a double-strand break. The repair after the damage will result in gene knockout or knock-in, etc., ultimately achieving the purpose of modifying the genomic DNA. Compared to traditional homologous recombination knockout methods, CRISPR-Cas9 gene-editing technology is faster, more trace-free, and more accurate, making it easier for you to conduct subsequent experimental studies. |
| Content |
Knockout
Knock-in
Point mutation |
Our advantages
- Fast experimental cycle, saving cost for customers
- Proven experimental technology for scarless genome editing
- Gene editing systems for multiple microorganisms, suitable for a wide range of customer projects
- Strong technical support for the development of CRISPR methods for new microorganisms
Lifeasible can rapidly and efficiently identify unknown gene functions and good functional genes through genetic modification of lactic acid bacteria. Most importantly, we can improve lactic acid strains for feed and fertilizer fermentation, and obtain more resistant lactic acid strains to improve the quality of fermentation products. If you are interested in our lactic acid bacteria gene editing service, please feel free to contact us. We will be happy to provide you with details of our lactic acid bacteria gene-editing project for fermentation.
For research use only, not intended for any clinical use.
