Bacillus subtilis is widely distributed in soil and decaying organic matter, without pods, with flagella, can move, Gram stain positive. The surface of the colony is rough and opaque, and it is an aerobic type of bacteria. Bacillus subtilis is fast-growing, has low nutritional requirements, secretes many proteins and metabolites efficiently, and does not produce toxins. It is a non-pathogenic and safe microorganism that has great potential for applications in the field of feed and fertilizer, such as in the fermentation process of feed and fertilizer production.
To achieve maximum productivity in production such as feed and fertilizer fermentation, Lifeasible can improve and streamline the genome of Bacillus subtilis using gene knockout, knock-in, and point mutation to obtain the optimal strain. We offer optimization strategies designed to maximize the production of target products while maintaining the growth of the strain itself by knocking out non-essential and redundant genes without significantly affecting the growth state of the strain. We can provide our customers with easy-to-use Bacillus subtilis that are free of resistance markers, with high transformation efficiency and high secretion capacity.
Our service approach
| Methods |
Details |
| Gene exchange methods |
We can use gene exchange and marker screening for Bacillus subtilis genome improvement and optimization, and we can apply cat, upp, blaI, mazF, ysbC, hewl, and other genes to reversible selection marker systems for Bacillus subtilis. |
| Tag-free recombinant technology |
We use the Cre/loxP system and the Xer/dif system to catalyze recombination between two specific recognition sites to produce the desired DNA integrations, deletions, or inversions that can be efficiently mutated on a genomic scale in Bacillus subtilis. |
| CRISPR/Cas9 technology |
We have developed a targeted CRISPR/Cas9 system for Bacillus subtilis for feed and fertilizer industry production. The system was used to disrupt specific genes in the Bacillus subtilis genome, and the resulting mutants formed less foam during fermentation of feed and fertilizer, showed greater resistance to spore formation, and secreted more active enzymes. |
Advantages of our technical services
- We have strain genetic manipulation technology characterized by easy operation, a high success rate, and high technical maturity.
- Control of synthetic production with Bacillus subtilis as the chassis cell can be performed according to your needs. Synthetic small molecule compounds or enzymes involved include riboflavin, surface activator, acetyl methyl carbinol, phytase, xylanase, L-asparaginase, chondroitin, N-acetyl glucosamine, etc.
- Gene inactivation, insertion, and targeted mutation can be achieved faster to optimize metabolic pathways, enhance metabolite production and achieve improved feed and fertilizer production efficiency.
Lifeasible offers efficient Bacillus subtilis strain transformation strategies that facilitate the regulation of target feed and fertilizer product production and allow you to rationalize and improve your production process promptly. We can improve the efficiency of the strain's synthesis of useful metabolites or target substances by knocking out non-essential genes in the heterologous synthesis pathway and the strain's metabolic coupling step during the transformation process. Please feel free to contact us. We welcome any questions you may have about how the modified Bacillus subtilis can be efficiently used in feed and fertilizer applications.
For research use only, not intended for any clinical use.
