Pichia pastoris is a methylotrophic yeast that is capable of growing on methanol as its sole carbon source. It has the advantages of tight regulation, high expression intensity, easy high-density fermentation, and the ability to perform protein post-modification. It has a wide range of applications in organic feed and fertilizer fermentation.
Lifeasible provides technical services to genetically engineer Pichia pastoris to meet the needs of the feed and fertilizer markets for the screening and selection of superior fermentation strains. We not only establish new metabolic pathways to enable strains to produce heterologous metabolites. We are also able to adjust the metabolic flux of the strains or delete some metabolic pathways, thus improving the production of homologous proteins or homologous metabolites. Making it better for specific feed and fertilizer fermentation processes.
We offer gene editing methods for Pichia pastoris
| Methods |
Details |
| Homologous recombination method |
We perform recombination in the form of a single exchange when gene integration is performed by plasmids. Recombination is performed as a double exchange when gene integration or gene knockout is performed by fragmentation. |
| Cre/loxP recombination method |
Allows for the elimination of conventional screening markers without toxicity to cells. |
| TALEN technology |
We enable gene editing through site-specific proteins and can perform targeted editing. |
| CRISPR/Cas9 technology |
Easy to use, we can select different conditions to achieve high gene editing efficiency (more than 80%) for Pichia pastoris. |
| Base editor |
It is a gene-editing technique based on CRISPR/Cas9 system without DSB. This technology allows us to perform single base mutations or stop codon mutations on the genome of Pichia pastoris. |
Service cases analysis
- We can improve Pichia pastoris using gene editing to make it highly acid-tolerant and can grow slowly at pH 2.
- We can modify Pichia pastoris by gene editing to produce high levels of ethyl acetate (with a unique apple aroma), which can be used in fermented feeds to increase palatability and feed intake.
- Our modified Pichia pastoris used in fermented feeds has greatly increased the protein content and hydrolytic enzymes of fermented feeds. This promotes animal digestion and absorption, improves feed utilization, and promotes animal growth.
Lifeasible provides gene-editing technology services for Pichia pastoris for feed and fertilizer fermentation purposes. We offer the advantages of trace-free editing, multiple gene editing (ability to knock out up to 3 genes at the same time), easy screening (no requirement for antibiotic resistance in the target bacteria), and fast cycle time. And we also offer custom services to develop special Pichia pastoris for better application in feed and fertilizer addition, fermentation, etc. Please feel free to contact us if you have a project that requires co-development. We look forward to working with you in this area.
For research use only, not intended for any clinical use.
