Fermented feeds are enriched with functional substances such as small peptides and organic acids through the degradation and transformation of macromolecules and anti-nutritional factors in feeds by microorganisms. Since different strains of bacteria have different physiological characteristics, researchers will choose the appropriate combination of strains to obtain the most optimal product according to different feed materials in production.
The diversity of bacteria involved in fermentation and the changes in the fermentation process will directly affect the quality of feed fermentation. Therefore, Lifeasible can provide customers with services for testing the bacterial flora in feed and fertilizer samples, including diversity analysis and analysis of dynamic changes during fermentation.
We offer services for the analysis of microbial diversity in samples
| Category |
Method |
Description |
| Biological or chemical-based methods |
- Traditional plate count method
- Fluorescent staining method
- Biolog microplate analysis
- Phospholipid fatty acid (PLFA) profiling
- Fatty acid methyl esters (FAME) profiling
These methods are based on the isolation and pure culture of microorganisms, and we determine the community structure by microscopic observation and physiological and biochemical characterization of pure microorganisms. |
| Methods based on modern molecular biology techniques |
Molecular labeling method |
- Fluorescence in situ hybridization
- Isotope labeling techniques
Using this method we can analyze the presence or absence, distribution pattern, and abundance of microorganisms in a specific environment with high sensitivity and specificity. |
| PCR-based methods |
- Random amplified polymorphic DNA (RAPD)
- Amplified restriction fragment polymorphism (AFLP)
- Restriction fragment length polymorphism (RFLP)
- Terminal restriction fragment length polymorphism (T-RFLP)
- Single-strand conformation polymorphism (SSCP)
- Ribosomal intergenic spacer analysis (RISA)
- Denaturing gradient gel electrophoresis (DGGE)
- Temperature gradient gel electrophoresis (TGGE)
Using these methods, we can amplify very small amounts of DNA in large quantities and study the diversity of microorganisms by comparative analysis of gene sequence specificity. |
| Methods based on DNA sequence determination |
- Metagenome sequencing technology
We can analyze the mutation of specific base sequences and find out the genetic evolutionary clues of microbial diversity by combining them with bioinformatics and performing a comparative analysis of data. |
Microbial change analysis service during the fermentation process
We can analyze the dynamic change of bacterial population during the fermentation of feed and fertilizer by measuring the growth curve and combining it with qPCR technology for single and mixed strains. We can provide accurate analysis for fermentation feed process optimization in terms of the evolution of the bacterial population.
Lifeasible provides a rapid, accurate, and specific method for analyzing large-scale mixed bacteria fermentation of bio-feed and fertilizer. If you have a sample that needs to be tested, please feel free to contact us for the latest solution.
For research use only, not intended for any clinical use.
