In the process of feed and fertilizer production, manufacturers pay attention to the influence of temperature, acidity, alkalinity, heavy metal ions, and other factors on the enzyme activity in the product, to achieve the best use of the product. Only feed and fertilizer products with optimized enzyme spectrum, stable enzyme activity, and good feeding and fertilizing effect can be more widely promoted in the market competition. Therefore, to meet the needs of manufacturers and researchers for enzyme activity determination in feed and fertilizer, Lifeasible offers a wide range of enzyme activity assays to our customers to meet your different testing needs.
The different kinds of enzyme activities we can test
| Type |
Methods |
| Glycosylase |
The activity is generally measured by the electrophoretic activity staining method and visible spectrophotometric method. |
| Protease |
Generally, the UV spectrophotometric method and forint method are used for determination. For the determination of alkaline protease activity, we generally use the fully automated biochemical analysis method. |
| Lipase |
Generally, potentiometric titration and indicator titration methods are used for determination. In addition, we also use dynamic titration to determine the enzymatic activity of lipase. For special lipases or special finished products at the sample preparation stage, we use special stabilization or extraction methods to ensure that all lipase activities are detected. |
| α-amylase |
Generally, medium temperature α-amylase activity and high temperature resistant α-amylase activity are determined by the colorimetric method and automatic biochemical analyzer method. |
| Phytase |
The spectrophotometric method (minimum quantitative limit of 130 U/kg) and molybdenum yellow method are generally used. In addition, we use a modified version of the method to determine phytase activity in feeds and fertilizers with lower detection limits. We eliminate the effect of inorganic phosphorus in feed and fertilizer on the hydrolysis reaction of phytase by reducing the content of inorganic phosphorus in feed and fertilizer samples by the pretreatment method. |
| Pectic enzyme |
The quantitative assay is generally performed by the hypo-iodite method. |
| Urease |
It is generally detected by the phenol red method and sodium hydroxide standard solution back-drop method. |
| β-glucanase |
It is generally detected by the spectrophotometric method and reducing sugar assay. |
| Superoxide dismutase (SOD) |
SOD activity was measured by the o-phenylene triol autoxidation method, hydroxylamine colorimetric method, xanthine oxidase-cytochrome C method, and chemiluminescence method. |
| Cellulase |
We have improved both the determination steps of cellulase activity and the assay of end products, thus reducing the complexity of sample processing and test steps, resulting in more accurate and reliable analytical results and better analytical reproducibility. The procedure is to use a fluorescent conjugate as a substrate for the assay and generate the fluorescent material by enzymatic digestion. This product is then fluorescently detected by a multifunctional enzyme standard and the cellulase activity involved in this reaction is calculated. |
| Xylanase |
The sensitivity of our modified ELISA method for micro detection of xylanase activity in feeds and fertilizers is 1×10-5 U/g. Our modified method not only detects xylanase activity in the samples but also has a high sensitivity. Moreover, because of its high sensitivity and the appropriate enzymatic digestion temperature used for the assay, the enzyme activity obtained by this method can be used to better evaluate the viability of xylanase in the samples. |
Lifeasible has mature technology and a well-developed testing process for enzyme activity testing of feed and fertilizer products. We can arrange the analysis as soon as possible after receiving the samples to be tested and provide you with the most professional and complete analysis report. We can meet your needs for product quality testing, evaluation, and product formulation development and improvement for feed and fertilizer products. If you have a sample that needs to be tested, feel free to contact us for the most suitable testing solution.
For research use only, not intended for any clinical use.
